Preclinical Models of Pain
Naason Science is working industriously to present new pain animal models. Pain, both acute and chronic, remains a significant health problem around the world despite tremendous progress in understanding of its basic mechanisms. There is still lot to be done. Naason Science animal models of pain are developed and designed to mimic distinct clinical diseases to better evaluate underlying mechanisms and potential treatments. That’s where the next generation of preclinical research begins.
Naason Science offers tools to study pain in rodents and larger animals. From in vitro systems to large and small animal in vivo models Naason Science provides a tool box of state-of-the-art technologies that allow for rapid and effective testing of preclinical drug candidates. Examples of Naason Neuropathic and Local Pain Models and Areas of Expertise:
• SC Ligation (Chung & CCI)
• Cpl& CPA-induced Visceral Pain
• Endometriosis Pain (female only)
• Post-operational Pain
• Trigeminal Neuralgia
Von Frey’s data generated from Naason’s Cisplatin Induced Peripheral Neuropathy Model (CIPN).
Endometriosis Model in Rats
Endometriosis is an estrogen-dependent inflammatory disorder characterized by the presence of endometrial tissue outside the uterine cavity. Patients experience chronic pelvic pain and infertility, with the most likely origin of the tissue deposits (lesions) being endometrial fragments shed at menses.
Naason Science autograft model of endometriosis has an 80 % rate of success in the implantation of autografts from donors to study groups and is confirmed by observation of stromal cells and epithelial cells in donors. The model can be used as a biochemical and histopathological model and also exhibits ongoing pain as well as anxiety and depression.
Intrastudy and study endpoint readouts from the model are:
- Inflammatory markers (TNF-alpha, IL1-Beta, Total antioxidant activity of test article (TAC)) etc.
- Continual core temperature in group housed rats
- Social distancing in the home cage
- Home cage locomotor activity in group housed conditions
- Animal postural changes
- Home cage rearing
- Ultrasonic vocalization (USV)
- Mechanical allodynia (von Frey) – including bladder
- Histopathlogical scoring (including mean volumes of inplants)
- Raloxifene as a control to gauge pelvic pain from endometriosis
- Plasma haematology and PCR
A typical paradigm for endometriosis in Naason’s laboratories:
Cyst formation
L5 Spinal Nerve Ligation (Chung Model) for Neuropathic Pain
-Spinal nerve ligation at level of L5
-Control compound Gabapentin
-8, 14 or 21 day studies
Intrastudy readouts:
- von Frey for mechanical allodynia
- Ultrasonic vocalization counts
- Homecage, group housed, rearing, locomotor activity
- Homecage social activity for anxiety and depression (social distance)
- Eating
- Drinking
- Grooming
- Body Weight
- Serum inflammary and neuro markers
- End-point tissue analysis
Ultrasonic Vocalizations Count - Group Housed HomeCage
Bladder pain (cystitis) CCP Model for Abdominal Pain
- Administration of the anticancer agent cyclophosphamide to induce bladder pain
- Morphine as a benchmark control
Intrastudy readouts include:
- Abdominal von Frey
- Ultrasonic vocalization (USV)
- Serum biomarkers for inflammation
- Home cage, group housed, locomotor and social activity
- Home cage rearing, eating, drinking, circadian rhythm, aggression and posture
- Home cage distance travelled and velocity
- Body Weight
End of study readouts:
- FACS
- SPECT
- Neuronal and Inflammatory mar
Ultrasonic Vocalizations Count - Group Housed HomeCage
We have also following Models available
- CCI-induced NP in rats
- Chung-induced NP in rats
- Cisplatin-induced NP
- Visceral pain model (Cyclophosphamide-induced bladder & hindpaw pain) in rats
- Chemotherapy (Cisplatin)-induced NP model
- Chemotherapy (Oxyliplatin)-induced NP model
- Endometriosis in mice
