In Vitro Models

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Naason Science offers a wide range of in vitro models designed to accelerate drug discovery and deepen understanding of disease mechanisms. Our in vitro platforms provide precise, controlled environments to study cellular processes, evaluate therapeutic efficacy, and identify potential drug targets. From cell-based assays to high-throughput screening systems, our models cover diverse research areas, including oncology, metabolic diseases, neurodegenerative disorders, and toxicology. With reliable and reproducible results, Naason Science’s in vitro models are an essential tool for bridging the gap between early-stage research and clinical development. 

• Primary Neuronal & Glial culture
• Osteoblast / Osteoclast culture 
• Angiogenesis culture 
• Lipid accumulation in HepG2 cells

Bone is in a constant state of remodeling, which is important for the maintenance of normal skeletal structure and function. Osteoclasts are responsible for aged bone resorption and osteoblasts are responsible for new bone formation. The resorption and formation is in stable at physiological conditions. However, when the balance is disturbed, bone architecture or function will be abnormal. Bone metabolism diseases, such as osteoporosis or osteopetrosis will occur.

Osteoblast – MC3T3 subclone 4

The MC3T3-E1 cell line established from newborn mouse calvaria is a well-known in vitro osteogenic model system and has been widely used in bone tissue engineering-related research. MC3T3-E1 cells display a sequential development pattern of proliferation and differentiation, resulting in calcified bone tissue similar to in vivo bone formation. The cells differentiate in accordance with the gene activation of osteoblast markers such as alkaline phosphatase (ALP), type I collagen and collagenase-1 (MMP-1), osteonectin (OPN), osteocalcin (OCN). 

Assay Analysis

• Cytotoxic assay: CCK-8, MTT
• Osteoblast differentiation: ALP assay
• Gene expression: RNA sequencing, qPCR
• Protein expression: Western blot, ELISA  

 Osteoclast are multinucleated bone resorbing cells formed by cytoplasmic fusion of their mononuclear precursors. The Bone marrow-derived macrophages (BMDM) are primary macrophages obtained by in vitro differentiation of bone marrow cells in the presence of macrophage colony-stimulating factor (M-CSF or CSF1) and RANKL.

Assay Analysis:

• Cytotoxic assay: CCK-8, MTT
• Osteoclast differentiation: TRAP stain
• Gene expression: RNA sequencing, qPCR 
• Protein expression: Western blot  

The in vitro lipid accumulation model using HepG2 cells is a valuable tool for studying non-alcoholic fatty liver disease (NAFLD) and related metabolic disorders. HepG2 cells, derived from human liver carcinoma, serve as a reliable platform for investigating lipid metabolism and the molecular mechanisms underlying steatosis. By inducing lipid accumulation in these cells, researchers can evaluate the efficacy of therapeutic compounds, explore pathways involved in lipid homeostasis, and identify potential targets for drug development. This model provides a cost-effective and scalable approach to advancing our understanding of liver-related metabolic diseases.